Objective: To observe the role of puerarin on the
proliferation of vascular smooth muscle cells(VSMC) induced by thrombin(T)
and the effect of puerarin on the c-fos and bcl-2 protein expression.Method: Cell number and cell cycle analysis using flow cytometry were adopted as two different indicators of effects on
proliferation of VSMC.Western blot was used to indicate the changes of c-fos and bcl-2 protein after 24 h of treatment of T and puerarin.Result: 1.5×10~(-5)～1.5×10~(-3) mol·L~(1) puerarin could significantly suppress this stimulation of VSMC proliferation and DNA
synthesis induced by T.Western blot demonstrated that after 24 hour of treatment with T and puerarin,T could significantly increase c-fos and bcl-2 protein and 1.5×10~(-5)～1.5×10~(-3) mol·L~(-1) puerarin could significantly suppress this increase.Conclusion: puerarin can suppress the proliferation and DNA synthesis of VSMC promoted by T.This inhibitory effects of puerarin are closely related with the suppression of c-fos and bcl-2 protein.