[Objective] To study the effect of Rhubarb on liver damage and liver regeneration in thioacetamide-induced fulminant hepatic failure(FHF) in rats.[Methods] The rats were randomly divided into normal group,model group,Rhubarb group and lyophilized bovine transfer factor control group. Medicines were given 1 ml/100 g respectively 3 days before hypodermically injected with thioacetamide(TAA) once a day.At the same time,the rats were hypodermically injected with TAA 600 mg·kg~(-1) twice for two days to induce the model of FHF except those in the normal group which were injected with the 0.85 % salt solution hypodermically.Forty-eight hours later,the blood was collected to measure ALT,AST,TB and parameters of liver regeneration such as liver mitotic index and proliferation cell nuclear antigen.[Results] Rhubarb ould obviously decrease ALT,AST,TB and improve liver mitotic index and proliferation cell nuclear antigen of rats with FHF,which has significant difference compared with model group and control group(P<0.05 or P<0.01).[Conclusion]Rhubarb can prevent the development of FHF and improve liver regeneration.