Objective To investigate the protection effect of epigallocatechingallate(EGCG)on the immune
function of dendritic cells(DCs) after UVB irradiation. Methods The monocytes were isolated from peripheral blood and cultivated into DCs with cytokines such as GM-CSF and IL-4. DCs were harvested after culture for 7days and irradiated by different dose of UVB.200μg /ml
EGCG was added in certain groups immediately after UVB irradiation.
T lymphocytes were co-cultured with DCs which were irradiated only by UVB or treated by both UVB
irradiation and EGCG. MTT assay was used to detect the ability of DCs stimulating
Lymphocytes to proliferate. Results UVB irradiation inhibited the ability of DCs stimulating lymphocyte proliferation in a dose-dependence. The
inhibition rate reached 100% when UVB dose was higher than 10mJ/cm2. The inhibition rate of DCs was improved to some degree, respectively after treated with 200μg/mL EGCG. Conclusion The irradiation of UVB inhibits cellular immune function of DCs directly. EGCG can antagonize such an immunosuppression induced by UVB irradiation.
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