ObjectiveTo study the effect and correlationship of
Xinzhi Bimin capsule on
expression of NF-κB and IL-5 in the spleen of
experimental allergic rhinitis(AR) in rat,and identify the mechanism of pharmacodynamic action.MethodsThe
expression of positive cell of NF-κB was detected in semiquantity by immunochemistry and the expression of IL-5 was detected in quantity by ELISA,which were compared with the different model rat established in AR and the normal control group.ResultsThe expression of the proportion of positive cell to NF-κB and IL-5 were lower in the experimental group than the model group(P<(0.05)) and the positive control group except the normal control group,moreover,the level of IL5 in the experimental group was positively correlated to the proportion of positive cell to NF-κB.ConclusionThe result suggests that the capsule of Xinzhi Bimin can reduce the infiltration of EOS and treat the symptom of AR in rat by down-regulating the activation of NF-κB,inhibiting the expression of cytokines of Th2(such as IL-5).