OBJECTIVE To study the thrombolytic effects of rNK on
coronary thrombosis induced by electrical stimulation,and the protective
effects on myocardial damage subjected to ischemia reperfusion in Chinese experimental
miniature swine.METHODS Endoarterium was injuried and coronary thrombi were formed gradually through direct electrical stimulation on the coronary artery of animals.Artery rechannelization was analyzed by coronary artery angiograph and multiple media graphic analysis.The experiments adopted epicardiogram mapping to measure the scope and degree of myocardial ischemia,quantitative histologic assay(nitroblue tetrazolium,N-BT stain) to determine the size ofmyocardial infarction.Serum creatine phosphokinase-MB(CK-MB) activity was detected.RESULTS R-NK 0.25～0.5 mg·kg~(-1) improved coronary thrombolysis,lessened the thrombi area,and affiliated artery rechannelization.Furthermore,r-NK alleviated the degree of myocardial ischemia(Σ-ST),narrowed the ischemic area and inhibited the CK-MB activity.CONCLUSION R-NK can inhibit
coronary thrombosis induced by electrical stimulation,improve thrombolysis,and alleviate myocardial damage subjected to ischemia reperfusion after artery rechannelizaiton.