ObjectiveTo investigate the effect and mech anism of prepared Radix Polygoni Multiflori (RPM) on the elevation of extracell
ular
hydroxyl radical in striatum of rats induced by intracerebral perfusion of
6-hydroxy dopamine (6-OHDA). MethodsCerebral microdia lysis was used to establish the model. Hydroxyl radical was captured by salicylic acid, and 2,3-d i hydroxy benzyl acid (2,3-DHBA) and 2,5-dihydroxy benzyl acid (2,5-DHBA) forme d b y hydroxyl radical in vital brain were measured by high performance liqui d chromatography-electrochemical detector (HPLC-ED). Results After perfu sion of 6-OHDA in brain of rats, the levels of 2,3-DHBA and 2,5-DHBA in the mod el group increased rapidly. The former was higher during the whole course of obs ervation (P<0.01), while the latter was higher at most time points than t hat in the control group (P<0.05 or P< 0.01). The level of 2,3-DHBA in the RPM group was lower than that in the model group at 5 time points (P <0.05, P<0.01). ConclusionRPM could inhibit th e elevating of extracellular hydro xyl radical in striatum of rats induced by intracerebral perfusion of 6-OHDA, i n dicating one of the brain protective mechanisms of RPM may be related to its anti-oxidation effect.