Object: Study on the resistant rates and
resistance mechanisms of
Klebsiella pneumoniae to cephalosporins isolated from West
China hospital.Methods: After
Klebsiella pneumoniae was collected、isolated and identified,a bacterial susceptibility test was examined by agar dilution method and the standard disk diffusion method,Nitrocefin method was used to screen of β-lactamase,a disc diffusion confirmatory test was used to detect ESBLs-producing strains as described in NCCLS2004,and three-dimensional test was used to detect strains which continuously producing high AmpC β-lactamase.Results: The resistant rate of the 18 strains Klebsiella pneumoniae to cefepime were 11.1% and 22.2% respectively, but the resistant rates to third generation cephalosporins was all higher from 22.2% to 66.7%;11 strains produced β-lactamase including 9 strains producing ESBLs and 1 strain producing AmpC enzyme.Conclusions: Production of β-lactamase is one of the
resistance mechanisms of Klebsiella pneumoniae to cephalosporins.