Aim To study release feature of tashinone Ⅱ A solid lipid nanoparticles(TA-SLN) in vitro,and clarify the differenc e in
absorption of TA-SLN from varied
intestinal segments and absorptive mechan ism in vivo.Methods Dialytic method was used to determine t ashinone Ⅱ A release rate of nanoparticles in vitro. An in situ rat perfusion method was used to investigate the intestinal
absorption of TA-SLN.Resu lts Tashinone Ⅱ A release from TA-SLN in vitro fit the Weibull e quation well and had a property of sustained release. The absorption rate of tas hinone Ⅱ A solution from varied intestinal segments had no significant differe nce(P>0.05). However,compared with others segments, significantly high per centage of TA-SLN was absorbed in colon(P<0.05). The absorption of tashino ne Ⅱ A tended to be saturated with the high concentration of TA-SLN. It did n ot increase significantly when blank nanoparticles were added to the perfusion s olution(P>0.05). With the decrease of Na+ or existence of deoxycholate, T ween 80,SLS or DNP in the perfusion solution, the absorption rate increased rem arkably(P<0.05). Conclusion TA-SLN could sustain to relea se drug. The colon was the most potentest site of TA-SLN absorption in intestin al tract.