Objects: To investgigate the influence of
Qingzhi capsule on platelet intraceller cAMP and cGMP levels,intraceller calcium
of platelet (
i), plasma PGE_2,TXB_2 and 6-Keto-PGF_~1α in two models of LPS attack in rats.Methods:Two kinds of model were established: after oral administration of Qingzhi capsule 8d, attack was induced by intraperitoneal injecting(i.p.)LPS; another model was established with high fat diet for 8d,then induced by i.p.LPS at the last day.At 6h or 24h of post-supplementation rats were anesthetized,blood was collected immediately from abdominal aorta.The levels of Plasma TXB_2 and 6-Keto-PGF_~1α was assayed by radioimmunoassay (RIA),cAMP and cGMP levels of platelet were assayed by RIA too.i were examined with flow cytometer.The level of Plasma PGE_2 was measured by enzyme-immunoassay(EIA).Results and Conclusion:Qingzhi Capsule increased the level of platelet i on 24h after LPS attack,Qingzhi Capsule improved the levels of Plasma PGE_2 and 6-Keto-PGF_~1α at 24h in the rats with only LPS attack.Qingzhi Capsule had strong effect on prevention of increasing TXB_2.Results also indicated depressing of TXB_2/6-Keto-PGF_~1α ratio in the rats with high fat diet and LPS attack.