Objective:To study the molecular mechanism of Anti fibrosis herbal compound against liver fibrosis,and to explore the feasibility
of its application in preventing and treating the portal hypertension.Methods:The serum of rats containing Anti fibrosis herbal compound drags was nade.
Hepatic stellate cells(HSCs) from rats were treatea with Anti fibrosis Formuia drug serum and carbon tetrachioride(CCl 4) for 24 hours respectively and loaded with Fluo 3/AM.The intracellular calcium ion(Ca 2+ ) level in each cell was determined with
laser scanning confocal microscopy(LSCM).Orthogonal design was used to study the influence on Ca 2+ in hepatic stellate cells treated with carbon tetrachloride,Anti fibrosisherbal compound serum,carbon tetrachloride and their order,but their time spans had no influence on Ca 2+ .Carbon tetrachloride caused obvious elevation of Ca 2+ when the dosage of CCl 4 from 5mmol/L to 15mmol/L,5% to 20% Anti fibrosis Formula serum decreased sharply the concentration of Ca 2+ .Conclusion:The mechanism of Anti fibrosis herbal compound against liver fibrosis and portal hypertension is related with its remission of Ca 2+ overloading.