To establish a RP-HPLC method for the analysis of pharmacokinetics on Picroside-Ⅰ in Rats,the plasma samples which had been
added Vc in it were diluted by acetonitrile to deposit protein. The agilent XDB C18 column (250 mm×4.6 mm,5 μm)was used as analytical column under room temperature. The mobile phase was a mixture of acetonitrile-water at a flow rate of 1.0 mL·min-1. The UV detection wavelength was 277 nm. It is resultsed that the calibration curve was linear in the range of (0.1-150) μg·mL-1(r=0.9994). The lowest detectable
concentration of Picroside Ⅰ was 25 ng·mL-1(S/N=3.4). The recovery of the methods were more than 90%, RSD of intra-day and inter-day were less than 10% respectively. After three injection dose of Picroside-Ⅰ, the concentration-time curves
administration were both confirmed to two-compartment open models in rats. It is conclused that the concentration-time curves administration were both confirmed to two-compartment open models. The method is accurate and reliable. It can be used for the pharmacokinetic studies of Picroside Ⅰ in rats plasma. The sharmacokinetics characteristics showed Picroside Ⅰ was metabolized rapidly in the rat, distr ibuted abroad, and eliminated soon.