Objective:to study the effect of berberine on the apoptosis and protein levels of survivin in
experimentally induced dysphasia
of gastric epithelia cells. Methods:first, make the apoptosis models of
gastric cancer by using MNNG method. And apply the prevention, small, medium and large dosages of berberine to experimental models to interfere with normal treatment. Then observe the ratio of occurrence of gastric epithelia under an electro-microscope. Furthermore,measure the ratio of apoptosis by using TUNEL as well as the express of survivin protein by the method of immunohistochemistry. Results:berberine can enhance apoptosis index (AI) in
experimentally induced dysphasia of gastric epithelia cells. After the berberine interference treatment to experimental groups, the ratio of gastric epithelia was obviously decreased comparing with model group(P<0.05).There was significantly difference between experimental groups and model group(P<0.01). There were remarkable difference between large dose group and model group(P<0.01).Conclusion:berberine can prevent and treat the epithelia of gastric cancer on experimental rats. Its mechanism is related to increased apoptosis ratio and decreased surviving gene.