Objective To investigate the expression of signal transducers and activations and transcription 3(STAT3)in protecting cardiacmyocyte
in children with
viral myocarditis and the effect of astragaluson on cellular proliferations.Methods Neonatal SD mice cardiac myocyte cultures are prepared with enzymatic digestion, and incubated. CVB_3 group was inoculated with coxsackieviruses B_3;astragalus group was inoculated after being infected with CVB_3; control group was treated with same dose DMSO. Western Blot was used to study the protein of STAT3 and P-STAT3.Cell viability was analyzed by MTT.CVB_3 mRNA was analyzed by RT-PCR.Results The protein concentration of STAT3 has not different(P>0.05). But the
activation of STAT3 was higher in CVB_3 group than control groups(P<0.01). And the protein of P-STAT3 in study group was lower than CVB_3 group(P<0.01), moreover the concentration of astragalus affected the activation of STAT3. In MTT, cell viability in astragalus group was higher than CVB_3 group(P<0.01).Conclusion Astragalus could block the viral mRNA duplication and its protective role is closely related to STAT3.