Objective To investigate whether pharmacological serum from the rats taking
radix salviae miltiorrhizae (RSM) can inhibit increase of
intracellular free calcium level in
hepatic stellate cells (HSCs). Methods A total of 32 healthy SD rats were divided into 4 groups. Two groups were modeled into hepatic fibrosis by 40% CCl 4 injection subcutaneously for 9 weeks and RSM were filled into the stomachs of one group (group A) but the same volume of saline into the other group (group B) twice a day for 6 d. Another two groups were only filled with RSM (group C) and saline (group D) for 6 d without modeling into hepatic fibrosis. The pharmacological serum was drawn from the inferior vena cava at 2 h after the last time of RSM or saline and used to culture HSCs at the concentration of 10% for 24 h. After HSCs were loaded with Fluo-3/AM, the effects of RSM on
i with laser scanning confocal microscopy were examined before and after angiotensin Ⅱ treatment. Results In the activated HSCs of groups A and C, i decreased significantly as compared with groups B and D (P<0.05), no matter whether treated with angiotensin Ⅱ or not. Conclusion RSM decreased i in activated HSCs, which may be one of important ways to block liver fibrosis.
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