Objective To study astragalosides injection inhibiting hepatocyte apoptosis after cardiopulmonary resuscitation (CPR) in rats .Methods Thirty Sprague Dawley male rates were randomly divided into 5 groups(n=6): sham (control group);experiment groups and astragalosides groups were allowed to reperfuse spontaneously for 6 hours and 24 hours after cardiopulmonary resuscitation. The temporospatial cellular reactions were assessed by terminal deoxynueleotidyltransfemse-mediated dutp-FITC nick endlabelins(TUNEL) and transmission electron microscope(TEM) in control group, experiment groups and astragalosides groups after cardiopulmonary resuscitation. Bcl-2, Bax and NF-κB protein expression were studied using immunocytochemistry in every group. Results Apoptopic hepatic parenchymal cells were obviously observed in livers subsequent of rats in every group. TUNEL - positive cells was (56.63±3.41)%, (47.53±2.15)% at 6-hour and 24-hour in experiment group,and(38.26±3.02)%, (28.59±2.31)%at 6-hour and 24-hour in astragalosides group,and (3.12 ±0.56)% in control group. Bcl-2 and NF-κB protein expression in astragalosides group were higher than those in experiment group,and Bax were lower. Conclusion Astragalosides injection has protective effect on hepatocyte apoptosis after CPR in rats.