AIM: To study the relation between prolon ge d survival of
skin allograft by chuan-ke-zhi (CKZ, drug of Chinese herbal) and C D4+CD25+ regulatory T cells (CD4+CD25+ Tr) in mice. METHODS: Skin allograft and isograft model in mice were establi shed and CKZ was administered by intraperitoneal injection. To observe its influ ence on survival of the graft, three color fluorescent staining together with fl ow cytometry was used to analyze the change of CD4+CD25+ Tr. RESULTS: The survival of skin allograft in CKZ
group was signifi cantly
prolonged compared to control group, (19.5±2.3) days and (10.2±2.2) days, respectively, P<0.01. The ratio of CD4+CD25+
Tr to CD4+ T cell s in peripheral blood of the recipient went up after allogeneic transplantation, reaching peak at 8 d and then descended, which came back to normal level in cont rol group at 13 d but still higher than normal in CKZ group at 23 d. The CD4+C D25+ Tr level had no significant change in either CKZ group or control group a fter syngeneic
transplantation (P>0.05). CONCLUSION: CKZ has an
effect of prolonging the survival of skin allograft. Enhancement of CD4+CD25+ Tr might be one of the mechanisms under lying its immunosuppressive effect.
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