OBJECTIVE To prevent the abuse of antibiotics and reduce the appearance of drug-resistant bacteria. METHODS Various methods were used to enhance the supervision of antibiotics, for instance, strengthen the leadership and fully exert the management function of leaders, establish rational management regulations to use antibiotics; strengthen the training of the doctors and nurses; increase the efficiency of culture and drug-sensitive test; stipulate the rules and principles for the use of antibiotics ;increase the supervision and strictly control the purchase of medicine; publish monthly the result of culturing the pathogenic bacteria and the allergic cases; implement strict inspection and supervision of the use of the antibiotics in hospital, and make a timely feedback of the inspection in various forms, and give certain economic punishment to the medical personal and the departments concerned. RESULTS The effective control of the abuse use of the antibiotics in hospital was obtained. CONCLUSIONS The comprehensive measures of the control of antibiotics abuse adopted in hospital have been proven effective.