Objective:To study the relationship between the
protective effect of shenfu parenteral solution (SF) on
myocardium in rats and HSP 70 .Methods:To establish general
ischemia reperfusion(I/R) model.Thirty-five healthy white rats were divided into five
groups randomly:sham operation group(group C),I/R30 group (including 2 groups),SF group(including 2 groups).After myocardial ischemia lasting for 40min,then reperfusing for 30min or 120min.To detect hemodynamic parameter,myocardium MDA content,SOD activity,HSP 70
expression and ultrastructure were examined.Results:Compare with I/R group:LVSP and ±dp/d/max rose up,however,LVEDP decreased( P <0.05) in SF group.After mycocardial ischemia lasting for 120min,SOD activity in SF group increased,MDA content decreased( P <0.05) and HSP 70 expression reinforced( P <0.05).Electron microscope showed myocardium injury was lighter in SF group than in I/R group.Conclusion:The SF protective effect on myocardium was connected with its anti-oxygen-derived free radicals and anti-lipid peroxiaation response,as well as with acceleration of HSP 70 expression.