ObjectiveTo study the effect of Danshensu (DSS) and Ligustrazine (TMZ), the extracts of Chinese herbs for promoting blood
circulation, on angiotensin Ⅱ(Ang Ⅱ) induced
myocardial hypertrophy and its related genes, and to explore the mechanisms of inhibitory effect. MethodsAdopting one-step method, the total RNA of myocardial cells was extracted by TRIzol reagent. Then the expression of ANP and β-actin mRNA, as symbol of myocardial cells, were detected by RT-PCR. ResultsMolecular biological research showed that AngⅡ could
significantly increase the expression of ANP mRNA in myocardial cells (P<0.01), which could be significantly inhibited by Losartan (P<0.01), both DSS and TMZ had the inhibitory effect (P<0.05). AngⅡ could increase β-actin mRNA expression in myocardial cells simultaneously, Losartan, DSS and TMZ could also significantly inhibit it (P<0.05). ConclusionThe effective ingredients of Chinese herbs for promoting blood circulation, DSS and TMZ, have the effect of inhibiting the hyper-expression of ANP and β-actin induced by AngⅡ, and preventing myocardial hypertrophy, therefore, it could be used to prevent and treat cardiomegaly.