Objective To establish the determination of 3,
29-dibenzoyl-karounitriol by HPLC for optimizing the slices of prepared Semen
Trichosanthis (ST). Methods With 3,
29-dibenzoyl-karounitriol as the marker, HPLC was used to determine the contents of 3,29-dibenzoyl-karounitriolis in different slices of prepared ST, such as ST, stir-baked ST, stir-baked ST with bran, clamshell powder, or honey, ST frosting, ST kernel, stir-baked kernel of ST, and shell of ST in the condition of chromatographic column: Polaris C_(18) column (250 mm×4.6 mm,5 μm), mobile phase: methanol-water (93∶7), detecting wavelengh: 230 nm, flow rate: 1.0 mL/min. Results 3,29-Dibenzoyl-karounitriol is in the better linear range to the peak area of 0.048 4—2.420 μg and the mean recovery is 97.06%. The order in different slices of prepared ST is: kernel of ST>stir-baked kernel of ST>ST>stir-baked ST>stir-baked ST with bran>stir-baked ST with clamshell powder>stir-baked ST with honey>ST frosting>shell of ST. Conclusion ST and stir-baked ST are better slices of prepared ST, the results are in an accordance with the status of ST in clinical application.