OBJECTIVE To investigate the effects of
clarithromycin and azithromycin on the permeability of (gatifloxacin) (GAT) across
the bacterial biofilm of
Pseudomonas aeruginosa. METHODS In vitro model of (P. aeruginosa) biofilm was established using 0.22 μm dimension medical micropore film. The permeability of GAT across bacterial biofilm was observed in modified Franz diffusion cell and the effects of 64 μg/ml
clarithromycin and azithromycin on it were measured by HPLC. RESULTS After seven days′ continuous cultivation, P. aeruginosa could establish well-distributed and compact biofilm in the surface of micropore film, in which the content of glycocalyx was (57.69±32.97)μg/cm~2 and the amount of viable organism was ((2.15×10~7)) CFU/cm~2; (gatifloxacin) could slowly penetrate bacterial biofilm and the equilibration was achieved in 7h. When combined with 64 μg/ml clarithromycin or azithromycin, the permeation rate of gatifloxacin on h3 to h6 significantly (increased.) CONCLUSIONS In vitro model of P. aeruginosa biofilm can be successfully established using medical micropore film as a supporter; macrolide drugs such as clarithromycin and azithromycin can significantly promote the permeability of GAT across bacterial biofilm.