Objective: To study the effects of different concentrations of Gen o n the
proliferation of RPE cells under anoxic culture
conditions. Methods: Two m odels of RPE cells were set up, one under anoxic
conditions and the other under normal conditions as control. The four groups of RPE cells in the first model w ere treated with Gen 0,50,100,200 μmol/L respectively. After 24 h, the A value of MTT of RPE cells was measured, and the expression of Ras mRNA was detected wi th RT-PCR. ELISA was used to detect 3-NT , and Xanthine Oxidase Enzymic method for the validity of SOD. Results: In anoxic environment, with stronger genistein , there was increased expression of Ras mRNA in the cells. Compared with the co ntrol goup there was a significantly bigger increase of 3-NT(P < 0.05),and a bet ter expression of SOD(P < 0.05). Compared with the RPE cells not treated with Ge n in the anoxic environment, the A value showed a significant increase in the RP E cells treated with Gen 100,200(P < 0.05), indicating a
proliferation of RPE ce lls in 24 h. Conclusion: Gen could improve the oxidative stress of RPE cells und er anoxic conditions and lead to the increased expression of Ras mRNA and the pr oliferation of RPE cells, which might be related to the ROS that resulted 3-NT increase.