Preliminary　Studies　on　Detection　of　Corticosteroid　Hormones　and　Their　Metabolites　in　Urine　and　Blood

In　order　to　study　the　effects　of　carbon　disu lfide(CS2)on　the　function　of　adrenal　gland s　and　the　feasibility　of　its　application　in 　biological　monitoring,urine　and　blood　leve ls　and　corticosteroid　hormones　and　their　me tabolites　and　their　changes　were　detected　w ith　high　performance　liquid　chromatography　 and　radioimmunoassay　for　the　workers　expose d　to　CS2　for　long　term　in　a　synthetic　fibe r　works.Results　showed　that　blood　and　urine 　levels　of　corticosteroid　hormones　and　thei r　metabolites　declined　in　the　workers　expos ed　to　CS2　at　the　air　concentrations　in　the 　workplace　exceeding　the　national　hygienic　 standard.Analysis　with　multiple　stepwise　r egression　revealed　that　changes　in　adrenal　 corticosteroid　hormones　correlated　with　CS 2　concentrations　in　workplace　air.It　sugges ts　that　urine　levels　of　corticosteroid　horm ones,including　free　cortisol,cortisone　and　 aldosterone　which　reflect　the　functional　st atus　of　adrenal　glands,and　their　metabolite s　can　be　used　as　indicators　for　nontraumatic　effects　in　biological　monitoring..