AIM To establish a stable cellular model and obtain a relatively homogenous cell population for our studies of ischemia
through a simple and effective episode. METHOD To investigate the molecular mechanism of neuronal ischemia, PC12 and NG108 15 cell lines were now popular in neuroscience research use. RESULT After 20 min exposed in a glucose free and hypoxia medium executed by Na 2S 2O 4(1,2,4 or 8 mmol·L -1 ), the MK801 binding or PC and NG cells was significantly higher than that in the control groups, while the binding activities of m , n
cholinergic receptors in the same cells decreased as compared to the control. Morphologic changes also showed cells dysfunction. Futhermore, pretreatment of APV markedly lowered the
NMDA receptor activities in the ischemic cells and the cells seemed better survival. CONCLUSION Ischemic model using Na 2S 2O 4 and glucose free medium may well build up the excessive activation of glutamate NMDA receptors and cholinergic dysfunction in cells.