Objective To examine the penetration rates of 7 antibiotics into the liver and pancreatic tissues. Method Kunming mice received intravenous or oral bolus of antibiotics. Drug concentrations in serum and hepatic and pancreatic tissues were measured dynamically by microbiological agar diffusion method. The MIC 90 of tested drugs for common causative pathogens was determined. The penetration rates(tissue concentration/serum concentration) and bactericidal indexes(tissue concentration/MIC, BI) were calculated. Result The drug penetration rates of azotreonam into hepatic and pancreatic tissues were: 472 9% and 82 7%, respectively, ceftizoxime 99 0% and 44 6%,ceftitriaxone 92 4% and 22 0%, cefoperazone 408 8% and 33 3%, ceftibuten 17 2% and 0,netilmicin 5 8% and 8 7%, and vancomycin 11 6% and 9 0%. Azotreonam, ceftizoxime, ceftitriaxone and cefoperazone showed high bactericidal indexes(>100) to enterobacteraceae both in the liver and pancreas, while netilmycin and vancomycin′s BI was <2. Conclusion Azotreonam, ceftizoxime, ceftitriaxone and cefoperazone are effective in the treatment of bacterial infections in the liver and pancreas.