The minimum inhibitory concentrations (MIC) of 16
antibiotics against Gram negative isolates from immunocompromised patients
in Wuhan were determined by agar dilution method and the susceptibility rates of the isolates to these
antibiotics were analyzed. A WHONET 3 computer software was used to analyse the results. The main organisms were P.aeruginosa (32.5%), E.coli (22.9%), Klebsiella (14.3%), Enterobacter (7.9%), Acinetobacter (6.1%), and Citrobacter (4.9%). It was found that most isolates possessed resistance to 16 antibiotics in various degree. 47.2% strains were resistant to more than 5 antibiotics. Among 16 antimicrobial agents, the suscepibility rate of the isolates to imipenem/cilastatin was the highest (95%), to amikacin (88%) and to ceftazidime (86%) followed. The MIC 50 (MIC 90 ) of imipenem/cilastatin, amikacin and ceftazidime were 0.5 (2), 2 (64) and 2 (32) μg/ml respectively. The results suggested a rational regimen for treatment of
Gram negative bacteria infections in immunocompromised patients.