Cyclophosphamide (Cy) could decrease the number of
peripheral WBC and induce the
hematopoiesis depression in dogs. In this
study, different doses of rhIL-3 were administrated subcutaneously to the Cy-treated dogs for seven days, and the changes of symptoms and general conditions,
peripheral blood cells and bone marrow cells were observed in treated dogs. The results showed that, after rhIL-3 treated, the general conditions improved dramatically, the decreasing degree of the peripheral WBC which was caused by using Cy was alleviated obviously and the duration of low-number WBC (lower than 50% of normal value) was shortened. It was also demonstrated that the bone marrow CFU-GM, CFU-E and BFU-E were promoted, the total number of bone marrow nucleated cell and the counts of platelet and reticulocyte were higher than PBS control group. These effects of rhIL-3 were more obviously in large dose group. It is suggested that rhIL-3 has a strong promoting effects on
hematopoiesis and could be used in treatment of hematopoiesis depression especially in the patients using Cy to cure cancer.