Effects of Ganoderma polysaccharide on production and mRNA expressions of IL1α and TNFα in murine

Interleukin1(IL1)α mRNA expression could be detected in murine splenocytes after 3h incubation,it reached the peak level at 4h and then declined to be undetected after 5h incubation.TNF(Tumor Necrosis Factor)α mRNA expression occured after 4h incubation,reached the peak level at 4.5h and disappeared gradually after 5.5h.Ganoderma polysaccharide (GLB7) in different final concentrations(5,10,20,40μg/ml) was added into the cultures at the beginning of incubation and then,the amount of mRNA expressions for IL1α and TNFα was determined after 4h and 4.5h respectively.It was found that GLB7 could promote the mRNA expressions for both IL1α and TNFα,the increase rates of mRNA expression for IL1α were 7.6%,67.3%,105.0% and 143.1%,and were for TNFα 9.9%,33.9%,45.9% and 75.8% respectively.After 8h incubation,GLB7 could increase the activity of IL1 in the supernatants by 1.4%,3.0%,6.7% and 8.1%,and raise the activity of TNFα by 3.0%,14.1%,28.2% and 35.7% after 12h incubation respectively.It is suggested that the enhancement effects of Ganoderma polysaccharide on the production and secretion of IL1 and TNFα in primary murine splenocyte culture are due to promoting of the mRNA expressions for IL1α and TNFα respectively.