In the present work, the effect of intrahippocampl microinjection of
noradrenaline(NA) and its receptors antagonists and
agonists on cellular immune functions were investigated in normal and adrenalectomy rat by determing the proaliferative activity of Con A_stimulated splenic lymphocytes in MTT mothed, the results showed: (1) In normal group, the prolifertative activity of ConA_Stimulated spenic lymphocytes were inhibited with microinjection NA and β 1_,β 2_adrenergic receptors agonists Dobutamine( Dob, 4μl, 6 0×10 -3 mol/L), Metaproternol(Met, 4μl, 8 0×10 -3 mol/L), compared with their intensity of effect, NA>Met>Dob; the immunosuppression effect induced by NA was partly hindered by α_and β_receptor antagonists, phentolamine(Phen, 4μl, 8 0×10 -3 mol/L) and propranolon( Prop, 4μl, 8 0×10 -3 mol/L), and the action of Prop was more evident (2)In adrenalectomy group, immunosuppression effect induced by NA was inconspicuousness, the results suggested that NA inhippocampus can inhibit distinctly cellular immune functions, which was predominantly mediated by β 2 adrenergic receptor with a minor contribution of β 1_an a_adrenergic receptors Moreover, keeping intact construction and function of adrenal gland have an important role in the efflect of NA on cellular immune function