Objective To investigate the
effect and its mechanism of “
JinSanE” on liver
fibrosis induced by carbon tetrachloride (CCl4) in
rats. Methods Thirty-six Wistar rats were divided into three groups: healthy controls, CCl4 induced cirrhotic rats, and CCl4 induced cirrhotic rats treated with “JinSanE”, which was begun at the fourth week after exposure to CCl4. “JinSanE” was given once a day. Rats were killed after the administration of “JinSanE” for 4 weeks. The anti-fibrosis effect was determined by the
expressions of connective tissue growth factor (
CTGF) and transforming growth factor-β 1 (TGF-β 1) in liver tissue. The CTGF and TGF-β 1 mRNA expressions were detected by RT-PCR, CTGF and TGF-β 1 were assessed after immunohistochemistry staining. The serum level of hyaluronic acid (HA) was determined by RIA, and the liver histopathology was observed with light and electronic microscopy. Results Compared with the CCl4 induced cirrhotic rats, the expressions of TGF-β 1 and CTGF were decreased in the liver of the rats which were given “JinSanE” (P<0.05 or P<0.01), the serum level of HA was decreased and the histological changes of fibrosis were improved significantly in the rats treated with “JinSanE”. Conclusion “JinSanE” had protective effect against liver injury, and it could inhibit liver fibrosis induced by CCl4 in rats. The effect might possibly be attributed to its anti-inflammatory activity, together with its effect of down-regulating CTGF and TGF-β 1, and suppressing the activation of hepatic stellate cells in rats.
More summaries about the Effect of “JinSanE” on the expression and localization of CTGF and TGF-β_1 mRNA in fibrotic liver in