Culture diversity is one of the important tourism resources of Jiuzhaigou nature reserve, and the protection of the culture diversity resource is of great significance to tourism sustainable development. This research intends to identify the influences of tourism development upon the Anduo Tibetan cultural diversity by the questionnaires to the residents at Jiuzhaigou and the domestic tourists. The questionnaire to the local residents covers seven aspects, including their housing styles, spoken and written languages, religious faith, diet, dressing and incomes, etc, and it aims to survey the status quo of cultural diversity of the local Anduo Tibetan, the changes of cultural diversity as well as the local residents' attitudes towards those changes. The questionnaires to the domestic tourists is their perceptions of the cultural diversity, such as "how do you feel the folkways of the minority nationalities in Jiuzhaigou", "during your tour in Jiuzhaigou, can you have a strong sense of the minority features", "do you think whether the tourism development will impact these minority features or not", et al.. These questions are designed to find out tourists' impressions of the status quo of the cultural diversity. The results of analyzing the data statistically suggest that changes have taken place in the local Anduo Tibetan's housing style, spoken and written languages, diet, dressing and religious faith, etc. The change percentages of the spoken Tibetan, diet, dressing and religious faith are 79.4%、84.5%、78.4% and 39.2%, respectively. For protecting culture diversity resource of Jiuzhaigou,it is recommended that we protect the living circumstance of the culture of the local Anduo Tibetan, emphasize on resident involved in tourism, and establish folk-custom sections thus realizing the tourist sustainable development.