Aim To study the quality standard of JKS lotion.Methods The Dahuang,rhubarb,Huangbai,XuChangQing,Boinpian in JKS lotion were
identified by TLC.The reference substances of rhubarb were Rhen,Emodin,
Chrysophanol.Developer were petroleum ether,ethyl acetate,formic acids(10∶3∶1).The reference substance of Cortex phellodendri was berberine hydrochloride.Developer were n-butyl alcohol-acetic acid-water(10∶1∶1).The reference substance of radix cynanchi paniculati was paeonol.Developer was cyclohexane and ethyl acetate(4∶2).The borneol selects the crude drugs of contrast for use.Developer were petrolenm ether and ethyl acetate(6∶2),HPLC was adopted to determine the contents of Rhen,Emodin,Chrysophanol in JKS lotion.With chromatographic column for Symmetry C 18 column(3.9×150mm,5μm).Symmetry C 18 in advance column(3.9×20mm),flow phase:methanol-water-phosphoric acid(82∶18∶0.1).The temperature of Column was 24℃.Detection wavelength was 432nm.The speed of flow was 1.0ml/min.Results JKS lotion and corresponding contrast product present the same distinguishes and reacts.The density of Rhen,Emodin,Chrysophanol in
JKS lotions difference in 7.60～38.00μg·ml -1 (r=0.999 4),1.75～8.75μg·ml -1 (r=0.999 9),2.4～12.00μg·ml -1 (r=0.999 9).The linear relationship is good.Their average recovery rate in JKS lotions:Rhen was 98.02%,RSD=1.78%.Emodin was 96.63%,RSD=1.20%;chrysophanol was 97.05%,RSD=1.15%,(n=5).Conclusions The method is sensitivly and selective.The quantitative date are reliable.This method offers the intact experiment basis of JKS lotion quality standard.