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THE EFFECT OF SINGLE KIND OF CHINESE MATERIA MEDICA ON OSTEOGENESIS GEN EXPRESSION IN CULTURED OSTEO Article Summary

Summary by : TsingHua
Visits : 19  words: 300   Published: March 20, 2004
This abstract was translated from 单味中药对成骨相关基因表达的影响
In order to compare the effects of single kind of common Chinese material medica for fracture healing (SKCMM) on the expression of osterogenesis gene in osteoblasts cultivation in vitro, RT-PCR was used to observe the effects of SKCMM-containing serum on the mRNA expressions of BMP-2, cbfal and IGF in cultivated osteoblasts. The results showed: ① among SKCMM, Pyritum was the best in promoting the mRNA expression of cbfal, the next in proper order were Radix Dipsaci, Radix Angelicae Sinensis, Resina Olibani, Myrrha and Eupolyphagaseu Steleophaga but Rhizoma Drynariae had no this function; ② Radix Dipsaci and Rhizoma Drynariae were the best in promoting the mRNA expression of BMP-2, the nest in proper order were Radix Angelicae Sinensis, Pyritum and Morrha, but Eupolyphagaseu Steleophagaand and Resina Olibani had no this function; ③ Morrha was the best in promoting the mRNA expression of IGF, the next in proper order were Radix Angelicae Sinensis, Pyritum and Radix Dipsaci, while Rhizoma Drynariae, Eupolyphagaseu Steleophaga and Morrha had no this function, suggesting that SKCMM could influence the expression of osteogenesis gene but different drugs has different target genes.

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