Aim:In order to research the mechanism of treating knee
osteoarthritis with guyanding recipe,we study the influence of iNOS
expression in articular cartilage of the rabbit knee
osteoarthritic model with guyanding recipe treatment by Using the immunohistochemistry and image analysis methods.Method:36 New Zealand rabbits were divided into three groups-guyanding group,acetaminophen group and untreated group at postoperation.All rabits were established the Hulth knee osteoarthritic model.The three groups were dealt with different therapy respectively and taken samples at postoperation 4,8 and 12 weeks by killing 4 rabbits each group.All samples were undertaken immunohistochemistry and image analysis methods in order to testing their iNOS expressing intensity.Result:The size of iNOS posityve expressing intensity of guyanding group was notable lower than those of acetaminophen group and the untreated group.Conclusion:iNOS took a key role in the osteoarthritic pathological process and Guyanding recipe can prevent and treat the knee
osteoarthritis by down-regulating the expression of iNOS.