To investigate if the different extract of Eclipta alba have different hepatoprotective. After the mice wrer orally administered 7 days with water decoction,50% alcoholic extract and ethyl acetate extract of eclipta alba,injected intraperitoneally 300mg/kg acetaminophen to induced liver acute injury.Then the blood serum of ALT and AST were determined.The results of experimental mice were compare with control and simple injured groud. Experimentions showed that the extract of Eclipta alba inhibited the elevation of aALT and sAST level.Compare with the simple injured groud,water decoction groud is no significant difference( P >0.05),50% alcoholic extract groud and ethyl acetate extract groud are significant difference( P < 0.05) and very significant difference( P <0.01),respectively. Different extract of Eclipta alba with different methods have different hepatoprotective effect on injured liver induced by acetaminophen in mice,and the hepatoprotective of the ethyl acetate extract of Eclipta alba is the best.