Objective:To develop a sensitive and simple reversed
phase liquid chromatography
method for analysis of
itopride hydrochloride in human
plasma Methods:Itopride hydrochloride in plasma was extracted with the mixture of ether and sodium hydroxide,then measured by HPLC using a nucleosil C 18 reverse phase column as stationary phase and acetonitrile phosphate buffer as mobile phase Itopride hydrochloride was quantified by ultraviolet absorbance at 257 nm
Results:The calibration curve was linear in the range from 10 ng·ml -1 to 1600 ng· ml -1 with a regression coefficient of 0 9995 and the lowest limit of detection of itopride hydrochloride in plasma was 10 ng·ml -1 The relative standard deviation was less than 7% both inter and intra batch,and the recoveries of itopride hydrochloride at three concentration levels of low,medium and high were 102 8%～106 3% The method was used to determine itopride hydrochloride in plasma from twenty volunteers Conclusion:The results of our study demonstrate that this method is rapid and accurate and it can be applied to
determination of plasma itopride concentration and to study on pharmacokinetics
More summaries about the RP-HPLC DETERMINATION OF ITOPRIDE HYDROCHLORIDE IN PLASMA