Objective:To establish the
method for
quality control of almond compound pill (Semen Armeniacae,Radix astragali,Radix asteris).Methods:Radix astragali and Radix
asteris were identified by TLC,and the content of amygdalin in Semen Armeniacae was determined by RP-HPLC.Kromasil C 18 column was used.The mobil phase consisted of methanol-aceton itrile-water-tetrahydro (14∶10∶75∶1)with flow rate 1.0ml·min -1 .The detection wavelength was set at 215nm.Results:The underneath Layer of chloroform-methanol-water(13∶7∶2)was
applied as the
developer for the identification of Astragaloside IV on silica gel G
coating plate.Thepetroleum(60～90℃)-ethyl acetate(9∶1)was applied as the developer for the identification of Radix asteris on silica gel G coating plate.The method was Liner within the range of 0.6～3.4μg (r=0.9997).The average recovery of assay was 98.34% and RSD was 0.73% (n=5).Conclusion:The method appeared to be simple and rapid,highly accurate and precise.It can control the quality of this preparation effectively.
More summaries about the Study on quality standard for almond compound pill