Objective To observe the effect of
protamine (P)on biological activities of stichopus japonicus acid mucopolysaccharide (SJAMP).Method The tests included coagulative time (capillary tube method),mortality,platelet (microscopic counting method),weight of spleen and
inhibition rate of tumor growth (murine solid tumor S180).Results The
mice were given
SJAMP 100 mg/kg im,the coagulative time was (8.90±1.85) min,the number of
platelet was (43.40±10.16)×10 5/mm 3,the weight of spleen was (125.68±27.98) mg/10 g and the inhibition rate of tumor growth was 32.56%.The mice were given SJAMP 100 mg/kg im,adding P 100 mg/kg ip,the coagulative time was (3.55±1.52) min (P<0.001),the number of platelet was (56.00±5.32 )×10 5/mm 3(P<0.01),the weight of spleen was (110.20±14.09) mg/10 g and the inhibition rate of tumor growth was 30.81%.The mice were given SJAMP 650 mg/kg im,the mortality were 9/20 and 11/20 (24 h and 48 h after the administration respectively).The mice were given SJAMP 650 mg/kg im adding P 160 mg/kg×2 ip,the mortality was 3/20 (P=0.034) and 5/20 (P=0.041,24 h and 48 h after the administration respectively).Conclusion P has antagonistical effect on the anticoagulative effect and platelet aggregation induced by SJAMP in mice,P has some protective effect on the lethality induced by SJAMP in mice.P has no influence on immunoenhancement and anticancer effect of SJAMP in mice.
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